|Species:|| Rhodococcus equi|
Goodfellow & Alderson 1977
Rhodococcus equi is a Gram-positive coccobacillus bacterium. The organism is commonly found in dry and dusty soil and can be important for diseases of domesticated animals (horses and goats). The frequency of infection can reach near 60 percent. R. equi is an important pathogen causing pneumonia in foals. Since 2008, it is known that R. equi can infect wild boar as well as domestic pigs. In addition, R. equi can infect humans. At risk groups are immunocompromised people, such as HIV-AIDS-patients or transplant recipients. Rhodococcus infection in these groups of patients resemble clinical and pathological signs of pulmonary tuberculosis.
Taxonomically, R. equi has been categorized as Corynebacterium equi, Bacillus hoagii, Corynebacterium purulentus, Mycobacterium equi, Mycobacterium restrictum, Nocardia restricta and Proactinomyces restrictus.
- Pigs (wild and domestic)
The most common route of infection in horses is likely via inhalation of contaminated dust particles. Inhaled virulent strains of R. equi are phagocytosed by alveolar macrophages. During normal phagocytosis, bacteria are enclosed by the phagosome, which fuses with the lysosome to become a phagolysosome. The internal environment of the phagolysosome contains nucleases and proteases, which are activated by the low pH of the compartment. The macrophage produces bacteriocidal compounds (e.g., oxygen radicals) following the respiratory burst. However, like its close relative Mycobacterium tuberculosis, R. equi prevents the fusion of the phagosome with the lysosome and acidification of the phagosome. Additionally, the respiratory burst is inhibited. This allows R. equi to multiply within the phagosome where it is shielded from the immune system by the very cell that was supposed to kill it . After about 48 hours, the macrophage is killed by necrosis, not apoptosis. Necrosis is pro-inflammatory attracting additional phagocytic cells to the site of infection, eventually resulting massive tissue damage.
All strains isolated from foals and the majority of human, cattle and pig isolates contain a large plasmid. This plasmid has been shown to be essential for infection of foals, and presumably plays a similar role for infection of other hosts, although this has not been established yet. Strains that lack the virulence plasmid are unable to proliferate in macrophages. This virulence plasmid has been characterised in detail from equine (horse) and porcine (pig) strains, although only the former has been functionally characterised . These circular plasmids consist of a conserved backbone that is responsible for replication and bacterial conjugation of the plasmid. This portion of the plasmid is highly conserved and found in non-pathogenic Rhodococci plasmids. In addition to the conserved region, the virulence plasmids contain a highly variable region that has undergone substantial genetic rearrangements, including inversion and deletions. This region has a different GC-content from the rest of the plasmid, and is flanked by genes associated with mobile genetic elements. It is therefore assumed to be derived from a different bacterial species than the backbone of the plasmid via lateral gene transfer.
The variable region of the virulence plasmid contain genes that are highly expressed following phagocytosis of R. equi by macrophages . It is believed that this variable region is a pathogenicity island that contains genes that are essential for virulence.
A hallmark of the pathogenicity island (PAI) is that many genes within it do not have homologues in other species. The most notable of these are the virulence associated protein (vap) genes. All foals infected with R. equi produce high levels of antibodies specific for vapA, the first vap gene to be characterised. Deletion of vapA renders the resulting strain avirulent . In addition to vapA, the pathogenicity island encodes a further five full length vap homologues, one truncated vap gene and two pseudo vap genes. The porcine pathogenicity island contains five full length vap genes, including the vapA homologue, vapB. In addition to these unique genes, the pathogencity island contains genes that have a known function, in particular two regulatory genes encoding the LysR-type regulator VirR and the response regulator Orf8. These two proteins have been shown to control expression of a number of pathogenicity island genes including vapA. Other genes have homology to transport proteins and enzymes. However, the functionality of these genes has not yet been established, nor how the proteins encoded within pathogenicity island subvert the macrophage.
- ↑ G. Muscatello, D. P. Leadon, M. Klay, A. Ocampo-Sosa, D. A. Lewis, U. Fogarty, T. Buckley, J. R. Gilkerson, W. G. Meijer, and J. A. Vázquez-Boland. (2007) Rhodococcus equi infection in foals: the science of 'rattles'. Equine Vet.J. 39:470-478. In: PMID 17910275
- ↑ Makrai, L. et al. (2008): Isolation and characterisation of Rhodococcus equi from submaxillary lymph nodes of wild boars (Sus scrofa). In: Vet Microbiol. PMID 18499361
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- Monika Venner und Erich Klug: Die Rhodococcus-equi-Pneumonie beim Fohlen: Diagnose, Therapie, Prophylaxe In: Pferde spiegel Nummer 4, 2005. Seiten 155-158 PDF
- J. Ashour and M. K. Hondalus: Phenotypic mutants of the intracellular actinomycete Rhodococcus equi created by in vivo Himar1 transposon mutagenesis. In: Journal of Bacteriology. Volume 185, Nummer 8, April 2003. Seiten 2644-2652.
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- A. Triskatis: Semiquantitative Bestimmung von Antikörpern gegen Rhodococcus equi in Serum und Klolostrum bei Stuten und Fohlen mittels ELISA und der Vergleich mit Befunden der Lungenuntersuchung PDF
- Letek M et al.: Evolution of theRhodococcus equivap Pathogenicity Island Seen through Comparison of Host-Associated vapA and vapB Virulence Plasmids. In: Journal of Bacteriology. Volume 190, Number 17, September 2008. 5797–5805.
- G. Muscatello, D. P. Leadon, M. Klay, A. Ocampo-Sosa, D. A. Lewis, U. Fogarty, T. Buckley, J. R. Gilkerson, W. G. Meijer, and J. A. Vázquez-Boland. (2007) Rhodococcus equi infection in foals: the science of 'rattles'. Equine Vet.J. 39:470-478.